Protein

Gene:

JARID1B (official gene symbol)

Other symbol:

JARID1B

CT family:

CT31

CT identifier:

CT31

Aliases from NCBI:

FLJ10538 , FLJ12459 , FLJ12491 , FLJ16281 , FLJ23670 , KDM5B , PLU-1 , PUT1 , RBBP2H1A

RefSeq :

NP_006609.3

Protein Names (UNIPROT)

Histone demethylase JARID1B

Cellular localization

Subcellular Localization	Cell type	Methodology	PMID
nucleus	breast cancer cell lines	IHC	12237901
nucleus	breast cancer cell lines	Indirect immunofluorescence	10336460

Protein function and interaction

JARID1 proteins are histone demethylases that belong to the ARID family of DNA binding proteins and act as transcriptional repressors. PLU-1/JARID1B specifically removes methyl groups from di- and trimethylated histone 3 lysine 4 (H3K4) and presents a restricted pattern of expression (Yamame et al., 2007 PMID: 17363312; Secombe & Eisenman, 2007 PMID: 17568193; Xiang et al., 2007 PMID: 18048344; Christensen et al., 2007 PMID: 17320161).

A yeast two-hybrid screen using PLU-1 as bait has identified two interacting proteins, brain factor-1 (BF-1) and paired box 9 (PAX9), both of which are developmental transcription factors (Tan et al., 2003 PMID: 12657635). Precipitation of AR (androgen receptor) was able to pull down JARID1B when both proteins were coexpressed (Xiang et al., 2007 PMID: 18048344). Co-immunoprecipitation assays provided an evidence that JARID1B (RBP2-H1) can bind to RB-1 (Roesch et al., 2005 PMID:15803180). Chromatin immunoprecipitation assays confirmed that PLU-1/JARID1B can interact with the promoters of the metallothionein genes (Scibetta et al., 2007 PMID: 17709396) and with the promoter of tumor suppressor genes, including BRCA1 (Yamame et al., 2007 PMID: 17363312). A phage display screen using JARID1B as bait identified hPc2 of the Polycomb group of protein (Zhou et al., 2009 PMID: 19336002).