Protein

Gene:

CRISP2 (official gene symbol)

Other symbol:

CRISP2

CT family:

CT36

CT identifier:

CT36

Aliases from NCBI:

CRISP-2 , GAPDL5 , MGC111136 , TPX1 , TSP1

RefSeq :

NP_003287.1

Protein Names (UNIPROT)

Cysteine-rich secretory protein 2 precursor

Cellular localization

Subcellular Localization	Cell type	Methodology	PMID
membrane and cytoplasm	pachytene spermatocyte	Indirect immunofluorescence	16759931
intra-acrosomal	spermatozoa	Indirect immunofluorescence	15734896

Protein function and interaction

CRISP2 (TPX1) protein is the component of the sperm acrosome that remains associated with sperm after capacitation and acrosome reaction, and is relevant for sperm-oocyte interaction (Busso et al., 2005 PMID: 15734896), through complementary sites on the egg surface (Cohen et al., 2008 PMID: 18649285).The Crisp (cysteine-rich secretory) domain on its own can regulate ion channel activity, providing compelling evidence for a role for CRISP2 in the regulation of Ca(2+) fluxes observed during sperm capacitation (by similarity) (Gibbs et al., 2006 PMID: 16339766). In mouse, yeast two-hybrid assays identified the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) as an interacting partner of CRISP2, raising the possibility that CRISP2 is a MAP3K11-modifying protein or, alternatively, that MAP3K11 acts to phosphorylate CRISP2 during acrosome development (Gibbs et al., 2007 PMID: 17377140). Another protein, gametogenetin 1 (GGN1) was also identified as a CRISP2-binding protein (Jamsai et al., 2008 PMID: 18502891).