1 |
09/14/2007 |
17868268 |
mRNA Profiling for Body Fluid Identification by Multiplex Quantitative RT-PCR. |
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An alternative approach to conventional protein-based body fluid identification is gene expression profiling analysis. In the present work, we report the development of sensitive and robust multiplex ...
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2 |
07/01/2007 |
17852044 |
Quantitative assessment of transition proteins 1, 2 spermatid-specific linker histone H1-like protein transcripts in spermatozoa from normozoospermic and asthenozoospermic men. |
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Spermatid-specific linker histone H1-like protein (HILS1), transition proteins 1 and 2 (TNP1 and TNP2), and protamines 1 and 2 (PRM1 and PRM2) contribute to considerable dense packing of spermatid chr...
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3 |
01/01/2007 |
17491138 |
Nuclear organization of the protamine locus. |
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The human protamine gene cluster consists of three tightly regulated genes, protamine 1 (PRM1), protamine 2 (PRM2) and transition protein 2 (TNP2). Their products are required to repackage the paterna...
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4 |
09/01/2006 |
16952514 |
Reciprocal translocation t(7;16)(q21.2;p13.3) in an infertile man. |
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OBJECTIVE: To report the first case of reciprocal translocation t(7;16)(q21.2;p13.3) associated with male factor infertility. DESIGN: Case report. SETTING: University genetics laboratory and universit...
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5 |
05/01/2006 |
16474017 |
Sperm nuclear histone to protamine ratio in fertile and infertile men: evidence of heterogeneous subpopulations of spermatozoa in the ejaculate. |
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Sperm protamine deficiency is observed in a subset of infertile men, suggesting that the relative histone to protamine ratio may be altered in the spermatozoa of these men. We measured the ratio of nu...
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6 |
03/01/2006 |
16871843 |
[Structure and role of protamines 1 and 2 in spermatogenesis and male infertility] |
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In the last decade the abnormalities in male infertility became the main problem of more than 50% couples. The main reasons of male infertility are abnormal gonadotropin releasing hormone (GnRH) secre...
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7 |
09/01/2005 |
16052476 |
A global but stable change in HeLa cell morphology induces reorganization of DNA structural loop domains within the cell nucleus. |
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DNA of higher eukaryotes is organized in supercoiled loops anchored to a nuclear matrix (NM). The DNA loops are attached to the NM by means of non-coding sequences known as matrix attachment regions (...
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8 |
08/11/2005 |
15939171 |
Multiplex mRNA profiling for the identification of body fluids. |
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We report the development of a multiplex reverse transcription-polymerase chain reaction (RT-PCR) method for the definitive identification of the body fluids that are commonly encountered in forensic ...
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9 |
05/01/2005 |
15849227 |
Cellular expression of protamine 1 and 2 transcripts in testicular spermatids from azoospermic men submitted to TESE-ICSI. |
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Testicular sperm extraction (TESE) combined with ICSI is used to treat azoospermia. However, the factors that influence the outcome of ICSI in this situation are ill-defined. We sought to investigate ...
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10 |
12/10/2004 |
15452126 |
Nuclear matrix interactions at the human protamine domain: a working model of potentiation. |
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The compact eukaryotic genome must be selectively opened to grant trans-factor access to cis-regulatory elements to overcome the primary barrier to gene transcription. The mechanism that governs the s...
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11 |
09/01/2004 |
15163613 |
Nucleoprotein transitions during spermiogenesis in mice with transition nuclear protein Tnp1 and Tnp2 mutations. |
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Chromatin remodeling during spermiogenesis is characterized by a series of nuclear protein replacements. Histones are replaced by transition nuclear proteins, which are in turn replaced by protamines....
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12 |
04/01/2004 |
14986146 |
Molecular cloning of protamine-2 and expression with aging in Japanese monkey (Macaca fuscata). |
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In order to isolate genes relating spermatogenesis with postnatal development and aging, we have attempted to obtain genes showing differences in expression in testis of Japanese monkeys ( Macaca fusc...
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13 |
10/01/2003 |
14756422 |
Conservation of the PRM1 --> PRM2 --> TNP2 domain. |
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In mouse and human, the genes encoding protamines PRM1, PRM2 and transition protein TNP2 are found clustered together on chromosome 16. In addition, these three genes lie in the same orientation to on...
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14 |
10/01/2003 |
14526123 |
Separation of spermatogenic cells from adult transgenic mouse testes using unit-gravity sedimentation. |
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During the final stage of spermatogenesis (i.e., spermiogenesis), round spermatids differentiate into mature spermatozoa. This transformation is mediated by a suite of nuclear packaging proteins. Thes...
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15 |
08/08/2003 |
12775710 |
The structural organization of sperm chromatin. |
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The packaging of the male haploid genome within the differentiating spermatid nucleus is facilitated by small basic nuclear proteins called protamines. Although the majority of the DNA in human sperm ...
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16 |
07/01/2003 |
12620939 |
Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice. |
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Cytokinesis is incomplete in spermatogenic cells, and the descendants of each stem cell form a clonal syncytium. As a result, a heterozygous mutation in a gene expressed postmeiotically affects all of...
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17 |
06/01/2003 |
12771233 |
Decreased protamine-1 transcript levels in testes from infertile men. |
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Infertile men exhibit an aberrant protamine-1 (Prm1) to protamine-2 (Prm2) ratio at both the mRNA and protein level. We therefore investigated whether male infertility could be related to the amount o...
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18 |
02/01/2003 |
12569175 |
Single nucleotide polymorphisms in the protamine-1 and -2 genes of fertile and infertile human male populations. |
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Although various genetic factors have been implicated in human male infertility, the causative genes for the different types of idiopathic male infertility have not been elucidated. Protamines, which ...
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19 |
12/01/2002 |
12524352 |
Accelerated protein evolution and origins of human-specific features: Foxp2 as an example. |
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Genes responsible for human-specific phenotypes may have been under altered selective pressures in human evolution and thus exhibit changes in substitution rate and pattern at the protein sequence lev...
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20 |
03/01/2002 |
11914920 |
Protamine-1 and -2 mRNA in round spermatids is associated with RNA-binding proteins. |
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RNA-binding proteins in round spermatids have previously been assigned to the coding sequence of Prm1- and Prm2-mRNA. To further characterize this protein-RNA interaction, prior to cDNA synthesis, mic...
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