Protein
Gene:
CTCFL (official gene symbol)Other symbol:
CTCFLCT family:
CT27CT identifier:
CT27Aliases from NCBI:
BORIS , CTCF-T , dJ579F20.2 , MGC163358RefSeq :
Protein Names (UNIPROT)
Transcriptional repressor CTCFL
Cellular localization
| Subcellular Localization | Cell type | Methodology | PMID |
|---|---|---|---|
| nucleus | developing spermatocytes | IHC | 16854382 |
| nucleus | embryo stem cells (ES) | Immunofluorescence | 18467432 |
| nucleolus, centrosome and cytoplasm | HeLa (cervical cancer), HCT116 (colorectal cancer) and MCF7 (breast cancer) cell lines | Immunofluorescence | 22676270 |
| centrosomes | keratinocyte | IF and confocal microscopy | 22676270 |
| nucleolus | keratinocyte | Western blot and Immunofluorescence | 22676270 |
| nucleus and cytoplasm | spermatocytes | in situ hybridization | 12011441 |
| nucleus | spermatogonia and spermatocytes | IHC | 22676270 |
| cytoplasm | spermatogonia, Leydig cells, and epithelial prostate cells | IHC | 16854382 |
| nucleolus | various cell lines | Immunofluoresce, confocal microscopy and 3D recons | 21811597 |
Protein function and interaction
BORIS acts as a transcriptional regulator, directing epigenetic reprogramming at CTCF target sites, both in normal development and in tumorigenesis (Loukinov et al., 2002 PMID: 12011441). SET1A, a H3K4 methyltransferase, and BAT3, a co-chaperone recruiter, were find to be binding partners for BORIS. It was proposed that BORIS acts as a platform upon which BAT3 and SET1A assemble and exerts effects upon chromatin structure and gene expression (Nguyen et al., 2008 PMID: 18765639). BORIS may have a role in apoptosis induction in tumor cells (Dougherty et al., 2008 PMID: 18355444). BORIS directly induces expression of CT20, TSP50. BORIS-induced expression of TSP50 is governed by accessibility and binding of BORIS to the TSP50 promoter (Kosaka-Suzuki et al., 2011 PMID:21659515). Boris may play a role in nucleolar function (Jones et al., 2011 PMID:21811597). Rosa-Garrido et al., 2012 (PMID: 22676270) suggested that BORIS may have a role in the regulation of the correct progression of the cell cycle and in the licensing of RNA transcription, since BORIS protein is present in sites of RNA transcription. BORIS plays a role in the epigenetic regulation of the suprabasin gene, SBSN (Gaykalova et al.,2012 PMID:22792300). BORIS has a growth inhibitory effect on normal and neoplastic cells and may act as a tumor suppressor (Tiffen et al., 2013 PMID:23553099).
