The figures shown in this section represent a standardized analysis of all reported CT-antigens in the same set of mRNA preparations from normal human tissues and a selection of human cancer cell lines. The analysis was done by RT-PCR using the primer pairs indicated for each gene. A total of 1.0 �g of RNA was reverse-transcribed into cDNA using the Omniscript RT kit (Qiagen, Valencia, CA) using oligo (dT)18 primers (Invitrogen, Carlsbad, CA). JumpStart REDTaq� ReadyMix-(Sigma Aldrich, St. Louis,MO) was used for amplification according to the manufacturer's instructions. Samples were amplified with a precycling hold at 95^oC for 3 min, followed by 35 specific cycles of denaturation at 95^oC for 15 seconds,annealing for 30 seconds (10 cycles at 60^oC, 10 cycles at 58^oC and 15 cycles at 56^oC) and extension at 72^oC for 30 seconds followed by a final extension step at 72^oC for 7 minutes. The RT-PCR products were run on 1.5% agarose gels and stained by ethidium bromide.
We have no attempted to provide a written description of each analysis since interpretation is straightforward. In general, a clear band indicates the presence of the corresponding mRNA in the preparation while the absence of a band indicates the opposite. Weak bands, indistinct bands, bands of an unexpected size, blurs etc are interpreted as experimental background, weak cross reactions and possible very low levels of gene expression.