mRNA expression
Gene:
MAGEA4 (official gene symbol)Other symbol:
MAGEA4CT family:
CT1CT identifier:
CT1.4Aliases from NCBI:
MAGE-41 , MAGE-X2 , MAGE4 , MAGE4A , MAGE4B , MGC21336Gene expression pattern ? : Testis-selective
High-throughput data (expression in cancer samples)
SAGE(short, 10 nt tags) Show
SAGE(long, 17 nt tags) Show
MPSS (short, 13 nt tags) Show
EST Show
Validation by Ludwig Institute of Cancer Research of mRNA expression in normal tissues and cell lines (RT-PCR)
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The figures shown in this section represent a standardized analysis of all reported CT-antigens in the same set of mRNA preparations from
normal human tissues and a selection of human cancer cell lines. The analysis was done by RT-PCR using the primer pairs indicated for each gene.
A total of 1.0 �g of RNA was reverse-transcribed into cDNA using the Omniscript RT kit (Qiagen, Valencia, CA) using oligo (dT)18 primers
(Invitrogen, Carlsbad, CA). JumpStart REDTaq� ReadyMix-(Sigma Aldrich, St. Louis,MO) was used for amplification according to the manufacturer's
instructions. Samples were amplified with a precycling hold at 95oC for 3 min, followed by 35 specific cycles of denaturation at 95oC
for 15 seconds,annealing for 30 seconds (10 cycles at 60oC, 10 cycles at 58oC and 15 cycles at 56oC) and extension at 72oC for 30 seconds followed by a final
extension step at 72oC for 7 minutes. The RT-PCR products were run on 1.5% agarose gels and stained by ethidium bromide. |
Published data of mRNA expression in normal tissues
ShowTissue | Methodology | PMID |
---|---|---|
Testis | Oligonucleotide microarray | 11751535 |
Testis | RT-PCR | 7927540 |
Muscle | qRT-PCR | 16774536 |
Keratinocytes | qRT-PCR | 17993289 |
Fetal keratinocytes | qRT-PCR | 17993289 |
Embryonic stem cells | c-DNA microarray | 16819296 |
Published data of mRNA expression in neoplasias
Hematologic malignacies Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
Monoclonal gammopathy of undetermined significance (MGUS) |
0/6 (0%) | RT-PCR | 10438702 | |
Myeloma |
0/2 (0%)(stage II) | RT-PCR | ||
Myeloma |
0/9 (0%)(stage I) | RT-PCR | ||
Myeloma |
5/27 (18%)(stage III) | RT-PCR | ||
Monoclonal gammopathy of undetermined significance (MGUS) |
3/6 (50%) | RT-PCR | 15761016 | |
Myeloma |
3/10 (30%) | RT-PCR | ||
Adult T-cell leukemia/lymphoma (ATLL) |
35/57 (61%) | RT-PCR | 22323448 |
Brain cancer Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
Medulloblastoma (Pediatric) |
2/11 (18%) | qRT-PCR | 18398575 | |
Glioma |
1/30 (3%) | RT-PCR | 18240144 | |
Medulloblastomas |
7/25 (28%) | RT-PCR | 18426187 | |
Glioma |
3/44 (7%) | RT-PCR | 22534618 | |
Meningioma |
0/26 (0%) | RT-PCR |
Esophageal cancer Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
Squamous cell carcinoma |
37/41(90%) | RT-PCR | 21613820 |
Head & Neck cancer Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
Squamous cell carcinoma |
Primary tumor | 34/57 (60%) | RT-PCR | 20715104 |
Hepatobiliary cancer Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
Hepatocellular carcinoma |
6/30 (20.0%) | RT-PCR | 15723723 | |
Hepatocellular carcinoma |
1/21 (4.8%) | RT-PCR | 12747756 | |
Hepatocellular carcinoma |
4/22 (18%) | RT-PCR | 10189127 | |
Hepatocellular carcinoma |
8/50 (16%) | RT-PCR | 10220740 |
Lung cancer Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
Adenocarcinoma |
4/22 (18%) | RT-PCR | 14512184 | |
Adenosquamous carcinoma |
1/1 (100%) | RT-PCR | ||
Large cell carcinoma |
1/6 (17%) | RT-PCR | ||
Small cell lung carcinoma |
2/4 (50%) | RT-PCR | ||
Squamous cell carcinoma |
4/13 (31%) | RT-PCR | ||
Adenocarcinoma |
34/118 (29%) | RT-PCR | 16299236 | |
Bronchioloalveolar / adenobronchioloalveolar carcinoma |
12/75 (16%) | RT-PCR | ||
Squamous cell carcinoma |
35/57 (61%) | RT-PCR | ||
Non small cell lung carcinoma |
6/33 (18%) | qRT-PCR | 17066423 | |
Adenocarcinoma |
3/35 (8%) | RT-PCR | 7622303 | |
Large cell carcinoma |
0/1 (0%) | RT-PCR | ||
Small cell lung carcinoma |
1/3 (33%) | RT-PCR | ||
Squamous cell carcinoma |
3/14 (21%) | RT-PCR | ||
Lung carcinoma |
4/12 (33.3%) | Nested RT-PCR | 12133624 | |
Adenocarcinoma |
25/38 (67%) | Oligonucleotide microarray | 17208331 | |
Squamous cell carcinoma |
10/14 (71%) | Oligonucleotide microarray | ||
Adenocarcinoma |
1/5 (20%) | RT-PCR | 16651421 | |
Squamous cell carcinoma |
5/5 (100%) | RT-PCR | ||
Non small cell lung carcinoma |
48/239 (20%) | RT-PCR | 19545928 | |
Adenocarcinoma |
7/29 (24%) | RT-PCR | 22138038 | |
Squamous cell carcinoma |
6/29 (21%) | RT-PCR | ||
Adenocarcinoma |
14/154 (9%) | RT-PCR | 22826471 | |
Non small cell lung carcinoma |
22/136 (16%) | RT-PCR | 23645764 |
Melanocytic lesion Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
"In situ" melanoma |
0/3 (0%) | RT-PCR | 10215777 | |
Benign melanocytic nevus |
0/10 (0%) | RT-PCR | ||
Dysplastic nevus |
0/14 (0%) | RT-PCR | ||
Melanoma |
Metastatic tumor | 0/3 (0%) | RT-PCR | |
Melanoma |
Primary tumor | 0/4 (0%) | RT-PCR | |
Benign nevocellular nevus |
0/12 (0%) | RT-PCR | 7591235 | |
Dysplastic nevus |
0/4 (0%) | RT-PCR | ||
Lentigo maligna |
0/4 (0%) | RT-PCR | ||
Melanoma |
Metastatic tumor | 32/145 (22%) | RT-PCR | |
Melanoma |
Primary tumor | 11/100 (11%) | RT-PCR | |
Melanoma |
8/47 (17%) | RT-PCR | 10890380 |
Ovarian cancer Show
Tumor Subtype | Sample type | Frequency | Methodology | PMID |
---|---|---|---|---|
Serous ovarian adenocarcinoma |
3/19 (16%) | RT-PCR | 8550240 |
Published data of mRNA expression in Cell Lines
ShowOrigin tissue | Cell Line ID | Frequency | Methodology | PMID |
---|---|---|---|---|
Adult keratinocyte cell line | NHEK | RT-PCR | 19358718 | |
Adult T-cell leukemia/lymphoma (ATLL) | MT-1 MT-4 MJ TL-Om1 TL-Su TCL-Kan HUT102 |
7/10 (70%) | RT-PCR | 22323448 |
Breast carcinoma | AlaBM | 1/13 (8%) | RT-PCR | 12836015 |
Head and Neck carcinoma | BB49-HNC | 1/1 (100%) | Oligonucleotide microarray | 11751535 |
Lung carcinoma- SCLC | OC1 | 1/1 (100%) | Oligonucleotide microarray | 11751535 |
Melanoma | MI10221 | RT-PCR | 7927540 | |
Melanoma | Sk-Mel-37 Sk-Mel-28 Malme-3M |
3/5 (60%) | RT-PCR | 18395366 |
Melanoma | LB1751-MEL LB373-MEL LB1448-MEL.2 LB583-MEL.6 LB1781-MEL |
5/9 (65%) | Oligonucleotide microarray | 11751535 |
Melanoma | WM115-A WM3130-A |
2/4 (50%) | RT-PCR | 18205182 |
Melanoma stem cells (MSC) | WM115-NA WM1972-NA WM3130-NA |
3/4 (75%) | RT-PCR | 18205182 |
Neuroblastoma | SK-N-MC |
RT-PCR | 21785609 | |
Oral squamous cell carcinoma | PCI13-11 PCI1-1 PCI52 PCI68-1 |
4/4 (100%) | RT-PCR | 19358718 |
Osteosarcoma | U-2 OS HOS MG-63 |
RT-PCR | 21785609 | |
Ovarian carcinoma | FraW KlHe |
2/6 (33%) | RT-PCR | 12836015 |
Pluripotent stem cells | SC5 SC7 |
RT-PCR | 21785609 | |
Rhabdomyosarcoma | A204v |
RT-PCR | 21785609 | |
Rhabdomyosarcoma (embryonal) | RD | RT-PCR | 21785609 | |
Sarcoma | SW872 | 1/7 (14%) | RT-PCR | 15298487 |
Thyroid cancer | TT | 1/1 (100%) | Oligonucleotide microarray | 11751535 |
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