1 |
10/10/2007 |
17928627 |
Identification and Characterization of RHOA-Interacting Proteins in Bovine Spermatozoa. |
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In somatic cells, RHOA mediates actin dynamics through a GNA13-mediated signaling cascade involving RHO kinase (ROCK), LIM kinase (LIMK), and cofilin. RHOA can be negatively regulated by Protein Kinas...
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2 |
10/01/2007 |
17551920 |
A yeast two-hybrid system using Sp17 identified Ropporin as a novel cancer-testis antigen in hematologic malignancies. |
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Since most intracellular proteins are expressed with their ligands, ligands of cancer-testis (CT) antigens may also be CT in their distribution. Applying Sperm protein 17 (Sp17) as the bait in a yeast...
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3 |
09/01/2007 |
17712481 |
Localization of AKAP4 and tubulin proteins in sperm with reduced motility. |
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AIM: To perform screening, related to A-kinase anchoring proteins 4 (AKAP4) and tubulin proteins, in spermatozoa with absent or severely reduced motility in order to detect the status of the fibrous s...
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4 |
12/01/2006 |
17081177 |
Transmission electron microscopy, immunocytochemical and fluorescence in situ hybridisation studies in a case of 100% necrozoospermia: case report. |
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We present the ultrastructural, functional and chromosomal analyses of sperm from an infertile man with a normal karyotype and 100% necrozoospermia. Tests for microbial infection showed the presence o...
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5 |
09/01/2006 |
16929165 |
Expression of cancer-testis antigens as possible targets for antigen-specific immunotherapy in head and neck squamous cell carcinoma. |
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Cancer-Testis (CT) antigens are by definition expressed in tumor but not in healthy tissue except testis and might represent ideal targets for antigen-specific immunotherapy. Here, we present the firs...
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6 |
01/01/2006 |
16177223 |
AKAP3 selectively binds PDE4A isoforms in bovine spermatozoa. |
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Cyclic AMP plays an important role in regulating sperm motility and acrosome reaction through activation of cAMP-dependent protein kinase A (PKA). Phosphodiesterases (PDEs) modulate the levels of cycl...
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7 |
10/01/2005 |
15980003 |
Gene deletions in an infertile man with sperm fibrous sheath dysplasia. |
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BACKGROUND: Asthenozoospermia may sometimes be related to genetic structural defects of the sperm tail detectable by transmission electron microscopy. Dysplasia of the fibrous sheath (DFS) is a geneti...
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8 |
07/01/2005 |
16009167 |
Fluorescence in situ hybridization and molecular studies in infertile men with dysplasia of the fibrous sheath. |
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OBJECTIVE: To perform fluorescence in situ hybridization (FISH) and molecular analysis in patients with the genetic sperm defect "dysplasia of the fibrous sheath" (DFS). DESIGN: Retrospective study. S...
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9 |
01/01/2005 |
15342355 |
Tyrosine phosphorylation of the a kinase anchoring protein 3 (AKAP3) and soluble adenylate cyclase are involved in the increase of human sperm motility by bicarbonate. |
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Mammalian testicular spermatozoa are immotile, thus, to reach the oocyte, they need to acquire swimming ability under the control of different factors acting during the sperm transit through the epidi...
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10 |
07/16/2004 |
15257753 |
Association of sperm protein 17 with A-kinase anchoring protein 3 in flagella. |
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BACKGROUND: Sperm protein 17 (Sp17) is a three-domain protein that contains: 1) a highly conserved N-terminal domain that is 45% identical to the human type II alpha regulatory subunit (RII alpha) of ...
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11 |
03/01/2004 |
14996943 |
Increased phosphorylation of AKAP by inhibition of phosphatidylinositol 3-kinase enhances human sperm motility through tail recruitment of protein kinase A. |
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Sperm motility is regulated by a complex balance between kinases and phosphatases. Among them, phosphatidylinositol 3-kinase (PI 3-kinase) has been recently suggested to negatively regulate sperm moti...
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12 |
01/01/2004 |
14618620 |
A-kinase anchoring protein 3 messenger RNA expression in ovarian cancer and its implication on prognosis. |
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A-kinase anchoring protein 3 (AKAP3) is a sperm protein and its expression appears to be restricted to the testis in normal adult tissues. We investigated AKAP3 mRNA expression in 20 normal ovaries an...
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13 |
06/01/2003 |
12606363 |
A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum. |
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The fibrous sheath is a unique cytoskeletal structure located in the principal piece of the sperm flagellum and is constructed of two longitudinal columns connected by closely spaced circumferential r...
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14 |
05/01/2003 |
12887088 |
How do sperm swim? Molecular mechanisms underlying sperm motility. |
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Motility is a characteristic function of the male gamete, which allows spermatozoa to actively reach and penetrate the female gamete in organisms with internal and external fertilization. Sperm motili...
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15 |
05/01/2003 |
12672126 |
Fibrous sheath of mammalian spermatozoa. |
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The fibrous sheath is a unique cytoskeletal structure surrounding the axoneme and outer dense fibers and defines the extent of the principal piece region of the sperm flagellum. It consists of two lon...
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16 |
02/01/2003 |
12653254 |
2P1, a novel male mouse cDNA specifically expressed during meiosis. |
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We screened a mouse germinal cell expression library with a probe derived from Sob1, a human testis-specific cDNA, and identified 2P1, a new mouse cDNA. A database search revealed that 2P1 was 91% ide...
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17 |
10/30/2001 |
11696326 |
Interaction of heterotrimeric G13 protein with an A-kinase-anchoring protein 110 (AKAP110) mediates cAMP-independent PKA activation. |
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Heterotrimeric G proteins and protein kinase A (PKA) are two important transmitters that transfer signals from a wide variety of cell surface receptors to generate physiological responses. The establi...
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18 |
05/18/2001 |
11278869 |
Identification of sperm-specific proteins that interact with A-kinase anchoring proteins in a manner similar to the type II regulatory subunit of PKA. |
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The cAMP-dependent protein kinase (PKA) is targeted to specific subcellular compartments through its interaction with A-kinase anchoring proteins (AKAPs). AKAPs contain an amphipathic helix domain tha...
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19 |
03/01/2001 |
11229805 |
Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath. |
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Dysplasia of the fibrous sheath (DFS) is characterized by male infertility, asthenozoospermia, and morphologically abnormal flagella that possess a severely malformed fibrous sheath. In many cases, DF...
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20 |
11/01/1999 |
10529264 |
FSP95, a testis-specific 95-kilodalton fibrous sheath antigen that undergoes tyrosine phosphorylation in capacitated human spermatozoa. |
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Protein tyrosine phosphorylation has been associated with both capacitation and motility of mammalian sperm. During capacitation, human spermatozoa undergo tyrosine phosphorylation of a characteristic...
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